milkmaid,
That's the deal with mine - most were at least 60 days post-partum; had not specifically seen them cycling - we do rotational grazing, with moves daily or sometimes twice daily - and usually, when they move, they're all about EATING, not mounting.
Last year - same group of cows, same time frame, we got 80% conception rate on 1 AI service on synchronized cows - bred on observed heats.
This year, not so good; glad we weren't doing that progeny-test breeding trial THIS year. Have seen 3 of the cows that we AIed return to heat this week - and one of those that I injected with Estrumate last week, was in heat yesterday - 7+ days out from when she got her injection - but, she was the last thing to calve this spring - 6 weeks behind everything else. May have been her first estrus cycle, and the pgf2 may have had no effect whatsoever.