best i have done on flushing is 12 #1's and 1 #3, set the cow up and flushed again and got 13 #1's and 2 unfertilitzed, transfered 2 frozen #1 got 0 preg, transfered 4 fresh #1 and got 2 preg. Flip side is flushed 1 cow 3 times got 2 #1's, flushed another cow 2 got 3 to transfer. All i can say is results vary.