g-h-BSEalabama, mad cow feed, and VPSPr PRIONPATHY

[flounder]

Saturday, August 14, 2010

BSE Case Associated with Prion Protein Gene Mutation (g-h-BSEalabama) and VPSPr PRIONPATHY


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Author Summary

Bovine spongiform encephalopathy (BSE or Mad Cow Disease), a transmissible spongiform encephalopathy (TSE) or prion disease of cattle, was first discovered in the United Kingdom in 1986. BSE is most likely the cause of a human prion disease known as variant Creutzfeldt Jakob Disease (vCJD). In this study, we identified a novel mutation in the bovine prion protein gene (Prnp), called E211K, of a confirmed BSE positive cow from Alabama, United States of America. This mutation is identical to the E200K pathogenic mutation found in humans with a genetic form of CJD. This finding represents the first report of a confirmed case of BSE with a potential pathogenic mutation within the bovine Prnp gene. We hypothesize that the bovine Prnp E211K mutation most likely has caused BSE in ''the approximately 10-year-old cow'' carrying the E221K mutation.


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P.9.21

Molecular characterization of BSE in Canada

Jianmin Yang1, Sandor Dudas2, Catherine Graham2, Markus Czub3, Tim McAllister1, Stefanie Czub1 1Agriculture and Agri-Food Canada Research Centre, Canada; 2National and OIE BSE Reference Laboratory, Canada; 3University of Calgary, Canada

Background: Three BSE types (classical and two atypical) have been identified on the basis of molecular characteristics of the misfolded protein associated with the disease. To date, each of these three types have been detected in Canadian cattle.

Objectives: This study was conducted to further characterize the 16 Canadian BSE cases based on the biochemical properties of there associated PrPres. Methods: Immuno-reactivity, molecular weight, glycoform profiles and relative proteinase K sensitivity of the PrPres from each of the 16 confirmed Canadian BSE cases was determined using modified Western blot analysis.

Results: Fourteen of the 16 Canadian BSE cases were C type, 1 was H type and 1 was L type. The Canadian H and L-type BSE cases exhibited size shifts and changes in glycosylation similar to other atypical BSE cases. PK digestion under mild and stringent conditions revealed a reduced protease resistance of the atypical cases compared to the C-type cases. N terminal- specific antibodies bound to PrPres from H type but not from C or L type. The C-terminal-specific antibodies resulted in a shift in the glycoform profile and detected a fourth band in the Canadian H-type BSE.

Discussion: The C, L and H type BSE cases in Canada exhibit molecular characteristics similar to those described for classical and atypical BSE cases from Europe and Japan. This supports the theory that the importation of BSE contaminated feedstuff is the source of C-type BSE in Canada. It also suggests a similar cause or source for atypical BSE in these countries.


http://www.prion2009.com/sites/default/ ... tracts.pdf


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Monday, August 9, 2010

National Prion Disease Pathology Surveillance Center Cases Examined (July 31, 2010)

http://prionunitusaupdate2008.blogspot. ... ology.html


Tuesday, August 03, 2010

Variably protease-sensitive prionopathy: A new sporadic disease of the prion protein

http://creutzfeldt-jakob-disease.blogsp ... pathy.html


Monday, August 9, 2010

Variably protease-sensitive prionopathy: A new sporadic disease of the prion protein or just more Prionbaloney ?

http://prionunitusaupdate2008.blogspot. ... pathy.html


please see full text ;


Saturday, August 14, 2010

BSE Case Associated with Prion Protein Gene Mutation (g-h-BSEalabama) and VPSPr PRIONPATHY


http://prionpathy.blogspot.com/2010/08/ ... otein.html





TSS

 

[Livestock-D]

Very interesting post flounder, yes BSE originated in the U.K. did you the United Kingdom has the most cases world wide? This is why the British beef producers have the most strictest regulations for livestock movements. This is their attempt to try and eradicate BSE, TSE, foot and mouth among other livestock diseases. Of course this is an uphill battle.

As you might know already, the USDA has begun talks and meetings to discuss a new NAIS which would be Animal Disease Traceability.
http://livestock-id.blogspot.com/2010/0 ... ility.html

 

[flounder]

Very interesting post flounder, yes BSE originated in the U.K. did you the United Kingdom has the most cases world wide? This is why the British beef producers have the most strictest regulations for livestock movements. This is their attempt to try and eradicate BSE, TSE, foot and mouth among other livestock diseases. Of course this is an uphill battle.

As you might know already, the USDA has begun talks and meetings to discuss a new NAIS which would be Animal Disease Traceability.
http://livestock-id.blogspot.com/2010/0 ... ility.html

Howdy there Livestock-D,


as a consumer, my opinion (if that matters), you need some sort of traceability for your livestock. I don't want to know anything about your family, your income, your kids, your sex life, nothing but to be able to trace that animal from farm to fork. I want to know whether or not if that animal has been fed animal protein, antibiotics, and if myself or my family do get sick, we should be able to trace that product. I don't see the problem. you can trace every part on your car, I can find out anything I want about you on the internet. why can't I do that with a cow?

let's review a few things about the NAIS, why we need it past and present. let's review first the few mad cows that were accidently found, birth cohorts, herd cohorts etc., and how efficient or NOT the traceability of those 4 animals were....oops, that's right, the first stumbling and staggering mad cow in Texas was sent straight to the render, did not pass GO(inspection), did not get $200.(prion rapid test), and of course, was never confirmed. THEN the second mad cow, the one that would never have been confirmed if not for an act of Congress and the Honorable Fong of the OIG, and scientist from the EU and the USA asking questions, meanwhile the BSE MRR was being finalized. Then after 7 months of sitting on the shelf, after a secret test had already turned up positive, but yet still 7 months no confirmation on a BSE positive test that by the BSE Red Books, it should have been a 48 hour turnaround on that test. Finally, the 2nd Texas mad cow was confirmed positive BSE. later termed atypical h-BSE. Then you had the Alabama mad cow, now termed g-h-BSEalabama, and then of course the mad cow old Luther capped in Washington state, the white one, that changed colors, and then was said to be a Canadian trader c-BSE mad cow. IT's all Canada's fault ;-(NOT)

Be sure to see the latest data on the typical and atypical cases of BSE and Scrapie and any human CJD TSE there from. This is down toward the bottom of the posting.

BSE, USDA, NAIS, AND TRACEABILITY

Let's look at how the USDA et al trace BSE aka mad cow cases, birth, and index herd cattle in the past (or rather how they could not trace them).

TEXAS MAD COW (h-BSE), that was finally tested and documented 7+ months after an act of Congress, and Scientist from all over the Globe questioning the testing methods and negative findings of this Texas mad cow. ...

TEXAS h-BSE MAD COW CASE THAT WAS FINALLY DOCUMENTED

Birth Cohort The owner of Farm A kept very few herd records; this made finding documentation on this cow's birth cohort difficult. The birth cohort, by definition, included all cattle born on the positive animal's birth premises within 1 year, before or after, the positive animal's date of birth. The index cow was approximately 12 years of age in November 2004, but there was no exact birth date in the herd records. A potential age range of 11 to 13 years was used to sufficiently cover the animal's most likely age. Using this range, all cattle born on the index premises between 1990 and 1995 were considered part of the birth cohort. In lieu of the owner's records, herd records from Veterinary Services' Generic Database (GDB) were used to compile a list of brucellosis calfhood vaccination (CV) tag numbers from the index herd that corresponded to animals to be included in the birth cohort. There were 121 animals identified through GDB as having been calfhood vaccinated on the index farm between 1991 and 1994. The owner of Farm A did not calfhood vaccinate after 1994. Moreover, calfhood vaccinates include only heifers. Therefore, the list of 121 animals was not a complete list of all birth cohorts. However the tracing that response personnel conducted on other COI was designed to account for the remainder of the birth cohorts.

Feed Cohort ...

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see full text ;


Friday, August 20, 2010

USDA: Animal Disease Traceability August 2010

http://naiscoolyes.blogspot.com/2010/08 ... ugust.html



kind regards,
terry

 

[Livestock-D]

Right back at you Flounder,
Very interesting facts, I guess we know the diseases exist and are real. The need for a traceability system is a dire need now!
(Knock on wood) there has not been a major epidemic. Imagine if there was an outbreak, and we could not trace back to the farm of origin, what would we do? destroy 100 million head of cattle, because it can't be traced? (Not a scare tactic just an example.) we know what the outcome would be from that situation...