Recommendations of only using it on heifers is for Dairy ONLY. Beef cows are fair game. One straw per insemination is enough. There are 2 kinds of straws produced. One for AI and another for Flushing. The straw for AI is packed with 2.1 million cells and the straws for Flushing are packed with 5.0 million. As of now, the only way to get a straw for Flushing purposes is to have it custom produced. All the straws sold by Select, ABS, Genex, etc are the 2.1 million dose and are for AI only.
When we Flush with sexed semen, we use 4 straws of the 5.0 million dose units. 2 the first breeding and then 2 the next. The key in a Sexed Semen flush is to breed her a few hours later than with conventional semen. Rather than breeding at 12 and 24 hours, we breed at 16 and 28 hours. These were the guidelines recommended to us by Sexing Technologies, who owns the technology, and it has worked well so far.