Menu
Forums
New posts
Search forums
What's new
New posts
New media
New media comments
New profile posts
Latest activity
Media
New media
New comments
Search media
Members
Current visitors
New profile posts
Search profile posts
Log in
Register
What's new
Search
Search
Search titles and first posts only
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Forums
Cattle Boards
NCBA, R-CALF, COOL, USDA (No Politics!)
Ranchers in Texas Need to Pay Close Attention to CWD TSE Prion
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Help Support CattleToday:
Message
<blockquote data-quote="flounder" data-source="post: 1815149" data-attributes="member: 3519"><p>"Currently, there is scientific evidence to suggest that CWD has zoonotic potential; however, no confirmed cases of CWD have been found in humans."</p><p></p><p>PART 2. TPWD CHAPTER 65. DIVISION 1. CWD</p><p></p><p>31 TAC §§65.82, 65.85, 65.88</p><p></p><p>The Texas Parks and Wildlife Commission in a duly noticed meeting on May 25, 2023 adopted amendments to 31 TAC §§65.82, 65.85, and §65.88, concerning Disease Detection and Response, without changes to the proposed text as published in the April 21, 2023, issue of the Texas Register (48 TexReg 2048). The rules will not be republished.</p><p></p><p>Currently, there is scientific evidence to suggest that CWD has zoonotic potential; however, no confirmed cases of CWD have been found in humans.</p><p></p><p><a href="https://www.sos.texas.gov/texreg/archive/June302023/Adopted%20Rules/31.NATURAL%20RESOURCES%20AND%20CONSERVATION.html#57" target="_blank">https://www.sos.texas.gov/texreg/archive/June302023/Adopted Rules/31.NATURAL RESOURCES AND CONSERVATION.html#57</a></p><p></p><p>17 DETECTION OF CHRONIC WASTING DISEASE PRIONS IN PROCESSED MEATS.</p><p></p><p>Rebeca Benavente1, Francisca Bravo1,2, Paulina Soto1,2, J. Hunter Reed3, Mitch Lockwood3, Rodrigo Morales1,2</p><p></p><p>1Department of Neurology, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, USA. 2Universidad Bernardo O'Higgins, Santiago, Chile. 3Texas Parks and Wildlife, Austin, USA</p><p></p><p>Abstract</p><p></p><p>The zoonotic potential of chronic wasting disease (CWD) remains unknown. Currently, there are no known natural cases of CWD transmission to humans but increasing evidence suggests that the host range of CWD is not confined only to cervid species. Alarmingly, recent experimental evidence suggests that certain CWD isolates can induce disease in non-human primates. While the CDC strongly recommends determining CWD status in animals prior to consumption, this practice is voluntary. Consequently, it is plausible that a proportion of the cervid meat entering the human food chain may be contaminated with CWD. Of additional concern is that traditional diagnostic techniques used to detect CWD have relatively low sensitivity and are only approved for use in tissues other than those typically ingested by humans. In this study, we analyzed different processed meats derived from a pre-clinical, CWD-positive free-ranging elk. Products tested included filets, sausages, boneless steaks, burgers, ham steaks, seasoned chili meats, and spiced meats. CWD-prion presence in these products were assessed by PMCA using deer and elk substrates. Our results show positive prion detection in all products. To confirm the resilience of CWD-prions to traditional cooking methods, we grilled and boiled the meat products and evaluated them for any remnant PMCA seeding activity. Results confirmed the presence of CWD-prions in these meat products suggesting that infectious particles may still be available to people even after cooking. Our results strongly suggest ongoing human exposure to CWD-prions and raise significant concerns of zoonotic transmission through ingestion of CWD contaminated meat products. </p><p></p><p>***> Products tested included filets, sausages, boneless steaks, burgers, ham steaks, seasoned chili meats, and spiced meats. </p><p></p><p>***> CWD-prion presence in these products were assessed by PMCA using deer and elk substrates. </p><p></p><p>***> Our results show positive prion detection in all products. </p><p></p><p>***> Results confirmed the presence of CWD-prions in these meat products suggesting that infectious particles may still be available to people even after cooking.</p><p></p><p>***> Our results strongly suggest ongoing human exposure to CWD-prions and raise significant concerns of zoonotic transmission through ingestion of CWD contaminated meat products. </p><p></p><p>=====</p><p></p><p>Efficient CWD-like transmission of splenic prions in cervidized transgenic mice: a probable diagnostic marker for CWD infection in humans</p><p></p><p>Xu Qi, Liuting Qing, Manuel Camacho, Ignazio Cali, Qingzhong Kong</p><p></p><p>Department of Pathology, Case Western Reserve University, Cleveland, USA</p><p></p><p>***> Such experimentally generated splenic "humanized" CWD prions (termed eHuCWDsp) appear indistinguishable from prions in the brain of sCJDMM1 patients on Western blot.</p><p></p><p>Transmission of prion infectivity from CWD-infected macaque tissues to rodent models demonstrates the zoonotic potential of chronic wasting disease.</p><p></p><p>Samia Hannaoui1,2, Ginny Cheng1,2, Wiebke Wemheuer3, Walter Schulz-Schaeffer3, Sabine Gilch1,2, Hermann Schatzl1,2 1University of Calgary, Calgary, Canada. 2Calgary Prion Research Unit, Calgary, Canada. 3Institute of Neuropathology, Medical Faculty, Saarland University, Homburg/Saar, Germany</p><p></p><p>***> Further passage to cervidized mice revealed transmission with a 100% attack rate. </p><p></p><p>***> Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. </p><p></p><p>****> The disease manifested as atypical in macaques and initial transgenic mouse transmissions, but with infectivity present at all times, as unveiled in the bank vole model with an unusual tissue tropism. </p><p></p><p>***> Epidemiologic surveillance of prion disease among cervid hunters and people likely to have consumed venison contaminated with chronic wasting disease</p><p></p><p>=====END </p><p></p><p><a href="https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true" target="_blank">https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true</a> </p><p></p><p>Transmission</p><p></p><p>10:00 - 11:40am Thursday, 1st June, 2023</p><p></p><p>Plaza Ballroom</p><p></p><p>Moderator Dr. Tracy Nichols</p><p></p><p>10:00 - 10:20am</p><p></p><p>Transmission of prion infectivity from CWD-infected macaque tissues to rodent models demonstrates the zoonotic potential of chronic wasting disease.</p><p></p><p>Samia Hannaoui1,2, Ginny Cheng1,2, Wiebke Wemheuer3, Walter Schulz-Schaeffer3, Sabine Gilch1,2, Hermann Schatzl1,2 1University of Calgary, Calgary, Canada. 2Calgary Prion Research Unit, Calgary, Canada. 3Institute of Neuropathology, Medical Faculty, Saarland University, Homburg/Saar, Germany</p><p></p><p>Abstract</p><p></p><p>We provide evidence by transmission experiments to different transgenic mouse models and bank voles that Cynomolgus macaques inoculated via different routes with CWD-positive cervid tissues harbor infectious prions. We used tissue materials from macaques inoculated with CWD to inoculate transgenic mice overexpressing cervid PrPC followed by transmission into bank voles. We used RTQuIC, immunoblot and PET blot analysis to assess brains, spinal cords, and tissues of the gastrointestinal tract (GIT) for the presence of prions. Our results show that macaque materials induced clinical disease in transgenic mice with low attack rates. Clinical mice did not display PrPSc in immunoblot, but showed low-levels of prion seeding activity. Further transmission into bank voles led to a 100% attack rate with typical PrPSc signature in immunoblot, and high-level prion seeding activity in brain, spinal cord and GIT tissues. Second passage studies led to 100% attack rate in voles inoculated with brain, spinal cord and small intestine material from first round animals, with shortened survival times indicating adaptation in the new host. </p><p></p><p>This shows that prions detected in GIT tissues are infectious and transmissible. </p><p></p><p>Further passage to cervidized mice revealed transmission with a 100% attack rate. </p><p></p><p>Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. </p><p></p><p>The disease manifested as atypical in macaques and initial transgenic mouse transmissions, but with infectivity present at all times, as unveiled in the bank vole model with an unusual tissue tropism. </p><p></p><p>***> Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. </p><p></p><p><a href="https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true" target="_blank">https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true</a></p><p></p><p>terry</p></blockquote><p></p>
[QUOTE="flounder, post: 1815149, member: 3519"] "Currently, there is scientific evidence to suggest that CWD has zoonotic potential; however, no confirmed cases of CWD have been found in humans." PART 2. TPWD CHAPTER 65. DIVISION 1. CWD 31 TAC §§65.82, 65.85, 65.88 The Texas Parks and Wildlife Commission in a duly noticed meeting on May 25, 2023 adopted amendments to 31 TAC §§65.82, 65.85, and §65.88, concerning Disease Detection and Response, without changes to the proposed text as published in the April 21, 2023, issue of the Texas Register (48 TexReg 2048). The rules will not be republished. Currently, there is scientific evidence to suggest that CWD has zoonotic potential; however, no confirmed cases of CWD have been found in humans. [URL]https://www.sos.texas.gov/texreg/archive/June302023/Adopted%20Rules/31.NATURAL%20RESOURCES%20AND%20CONSERVATION.html#57[/URL] 17 DETECTION OF CHRONIC WASTING DISEASE PRIONS IN PROCESSED MEATS. Rebeca Benavente1, Francisca Bravo1,2, Paulina Soto1,2, J. Hunter Reed3, Mitch Lockwood3, Rodrigo Morales1,2 1Department of Neurology, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, USA. 2Universidad Bernardo O'Higgins, Santiago, Chile. 3Texas Parks and Wildlife, Austin, USA Abstract The zoonotic potential of chronic wasting disease (CWD) remains unknown. Currently, there are no known natural cases of CWD transmission to humans but increasing evidence suggests that the host range of CWD is not confined only to cervid species. Alarmingly, recent experimental evidence suggests that certain CWD isolates can induce disease in non-human primates. While the CDC strongly recommends determining CWD status in animals prior to consumption, this practice is voluntary. Consequently, it is plausible that a proportion of the cervid meat entering the human food chain may be contaminated with CWD. Of additional concern is that traditional diagnostic techniques used to detect CWD have relatively low sensitivity and are only approved for use in tissues other than those typically ingested by humans. In this study, we analyzed different processed meats derived from a pre-clinical, CWD-positive free-ranging elk. Products tested included filets, sausages, boneless steaks, burgers, ham steaks, seasoned chili meats, and spiced meats. CWD-prion presence in these products were assessed by PMCA using deer and elk substrates. Our results show positive prion detection in all products. To confirm the resilience of CWD-prions to traditional cooking methods, we grilled and boiled the meat products and evaluated them for any remnant PMCA seeding activity. Results confirmed the presence of CWD-prions in these meat products suggesting that infectious particles may still be available to people even after cooking. Our results strongly suggest ongoing human exposure to CWD-prions and raise significant concerns of zoonotic transmission through ingestion of CWD contaminated meat products. ***> Products tested included filets, sausages, boneless steaks, burgers, ham steaks, seasoned chili meats, and spiced meats. ***> CWD-prion presence in these products were assessed by PMCA using deer and elk substrates. ***> Our results show positive prion detection in all products. ***> Results confirmed the presence of CWD-prions in these meat products suggesting that infectious particles may still be available to people even after cooking. ***> Our results strongly suggest ongoing human exposure to CWD-prions and raise significant concerns of zoonotic transmission through ingestion of CWD contaminated meat products. ===== Efficient CWD-like transmission of splenic prions in cervidized transgenic mice: a probable diagnostic marker for CWD infection in humans Xu Qi, Liuting Qing, Manuel Camacho, Ignazio Cali, Qingzhong Kong Department of Pathology, Case Western Reserve University, Cleveland, USA ***> Such experimentally generated splenic "humanized" CWD prions (termed eHuCWDsp) appear indistinguishable from prions in the brain of sCJDMM1 patients on Western blot. Transmission of prion infectivity from CWD-infected macaque tissues to rodent models demonstrates the zoonotic potential of chronic wasting disease. Samia Hannaoui1,2, Ginny Cheng1,2, Wiebke Wemheuer3, Walter Schulz-Schaeffer3, Sabine Gilch1,2, Hermann Schatzl1,2 1University of Calgary, Calgary, Canada. 2Calgary Prion Research Unit, Calgary, Canada. 3Institute of Neuropathology, Medical Faculty, Saarland University, Homburg/Saar, Germany ***> Further passage to cervidized mice revealed transmission with a 100% attack rate. ***> Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. ****> The disease manifested as atypical in macaques and initial transgenic mouse transmissions, but with infectivity present at all times, as unveiled in the bank vole model with an unusual tissue tropism. ***> Epidemiologic surveillance of prion disease among cervid hunters and people likely to have consumed venison contaminated with chronic wasting disease =====END [URL]https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true[/URL] Transmission 10:00 - 11:40am Thursday, 1st June, 2023 Plaza Ballroom Moderator Dr. Tracy Nichols 10:00 - 10:20am Transmission of prion infectivity from CWD-infected macaque tissues to rodent models demonstrates the zoonotic potential of chronic wasting disease. Samia Hannaoui1,2, Ginny Cheng1,2, Wiebke Wemheuer3, Walter Schulz-Schaeffer3, Sabine Gilch1,2, Hermann Schatzl1,2 1University of Calgary, Calgary, Canada. 2Calgary Prion Research Unit, Calgary, Canada. 3Institute of Neuropathology, Medical Faculty, Saarland University, Homburg/Saar, Germany Abstract We provide evidence by transmission experiments to different transgenic mouse models and bank voles that Cynomolgus macaques inoculated via different routes with CWD-positive cervid tissues harbor infectious prions. We used tissue materials from macaques inoculated with CWD to inoculate transgenic mice overexpressing cervid PrPC followed by transmission into bank voles. We used RTQuIC, immunoblot and PET blot analysis to assess brains, spinal cords, and tissues of the gastrointestinal tract (GIT) for the presence of prions. Our results show that macaque materials induced clinical disease in transgenic mice with low attack rates. Clinical mice did not display PrPSc in immunoblot, but showed low-levels of prion seeding activity. Further transmission into bank voles led to a 100% attack rate with typical PrPSc signature in immunoblot, and high-level prion seeding activity in brain, spinal cord and GIT tissues. Second passage studies led to 100% attack rate in voles inoculated with brain, spinal cord and small intestine material from first round animals, with shortened survival times indicating adaptation in the new host. This shows that prions detected in GIT tissues are infectious and transmissible. Further passage to cervidized mice revealed transmission with a 100% attack rate. Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. The disease manifested as atypical in macaques and initial transgenic mouse transmissions, but with infectivity present at all times, as unveiled in the bank vole model with an unusual tissue tropism. ***> Our findings demonstrate that macaques, considered the best model for the zoonotic potential of prions, were infected upon CWD challenge, including the oral one. [URL]https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true[/URL] terry [/QUOTE]
Insert quotes…
Verification
Post reply
Forums
Cattle Boards
NCBA, R-CALF, COOL, USDA (No Politics!)
Ranchers in Texas Need to Pay Close Attention to CWD TSE Prion
Top