Menu
Forums
New posts
Search forums
What's new
New posts
New media
New media comments
New profile posts
Latest activity
Media
New media
New comments
Search media
Members
Current visitors
New profile posts
Search profile posts
Log in
Register
What's new
Search
Search
Search titles and first posts only
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Forums
Cattle Boards
Breeding / Calving Issues
ET protocols
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Help Support CattleToday:
Message
<blockquote data-quote="JustSimmental" data-source="post: 890785" data-attributes="member: 14624"><p>Synchronization Protocols for embryo transfer (fresh to fresh) is very similiar to setting up cattle for AI, but for one additional step - setting up the Donor.</p><p></p><p>Superovulation</p><p></p><p>The donor cow is treated with a gonadotropin called follicle stimulating hormone (FSH).</p><p></p><p>This hormone is given am and pm for four days and as a result of the treatment, multiple follicles should develop on the ovaries of the donor.</p><p></p><p>Multiple numbers of eggs will be released at estrus. This process is called superovulation.</p><p>Stages of Embryo Development</p><p></p><p>Grade 1: Excellent or Good. Symmetrical and spherical embryo mass with individual blastomeres (cells) that are uniform in size, color, and density.</p><p></p><p>Grade 2: Fair, irregularities in overall shape of the embryonic mass or in size, color, and density of individual cells.</p><p></p><p>Grade 3: Poor. Major irregularities in shape of the embryonic mass or in size, color, and density of individual cells.</p><p></p><p>Grade 4: Dead or degenerating. Degenerating embryos: non-viable.</p><p></p><p></p><p>1 Unfertilized</p><p></p><p>2 2 to 12-cell</p><p></p><p>3 Early Morula</p><p></p><p>4 Morula</p><p></p><p>5 Early Blastocyst (excellent)</p><p></p><p>6 Blastocyst (excellent)</p><p></p><p>7 Expanded Blastocyst</p><p></p><p>8 Hatched Blastocyst</p><p></p><p>9 Expanded Hatched Blastocyst</p><p>Protocol</p><p></p><p>Day 0 Insert CIDR</p><p></p><p>DAY 2 GNRH</p><p></p><p>Day 4 FSH</p><p></p><p>Day 5 FSH</p><p></p><p>Day 6 FSH</p><p></p><p>Day 7 FSH; PGF2alpha; Pull CIDR</p><p></p><p>Day 8 None</p><p></p><p>Day 9 Breed</p><p></p><p>Day 10 Breed</p><p></p><p>Day 16 Flush</p><p></p><p>Recipient cow's on a fresh to fresh flush need to be at or past standing heat by CIDR synchronization by day 16+, so Day 7 of superovulation for the donor cow / recip cows must be at day 0. </p><p></p><p>JS</p></blockquote><p></p>
[QUOTE="JustSimmental, post: 890785, member: 14624"] Synchronization Protocols for embryo transfer (fresh to fresh) is very similiar to setting up cattle for AI, but for one additional step - setting up the Donor. Superovulation The donor cow is treated with a gonadotropin called follicle stimulating hormone (FSH). This hormone is given am and pm for four days and as a result of the treatment, multiple follicles should develop on the ovaries of the donor. Multiple numbers of eggs will be released at estrus. This process is called superovulation. Stages of Embryo Development Grade 1: Excellent or Good. Symmetrical and spherical embryo mass with individual blastomeres (cells) that are uniform in size, color, and density. Grade 2: Fair, irregularities in overall shape of the embryonic mass or in size, color, and density of individual cells. Grade 3: Poor. Major irregularities in shape of the embryonic mass or in size, color, and density of individual cells. Grade 4: Dead or degenerating. Degenerating embryos: non-viable. 1 Unfertilized 2 2 to 12-cell 3 Early Morula 4 Morula 5 Early Blastocyst (excellent) 6 Blastocyst (excellent) 7 Expanded Blastocyst 8 Hatched Blastocyst 9 Expanded Hatched Blastocyst Protocol Day 0 Insert CIDR DAY 2 GNRH Day 4 FSH Day 5 FSH Day 6 FSH Day 7 FSH; PGF2alpha; Pull CIDR Day 8 None Day 9 Breed Day 10 Breed Day 16 Flush Recipient cow's on a fresh to fresh flush need to be at or past standing heat by CIDR synchronization by day 16+, so Day 7 of superovulation for the donor cow / recip cows must be at day 0. JS [/QUOTE]
Insert quotes…
Verification
Post reply
Forums
Cattle Boards
Breeding / Calving Issues
ET protocols
Top