Subject: Clinical Observations of BSE Infection in Red Deer
Date: October 4, 2007 at 9:05 am PST
P04.80
Clinical Observations of BSE Infection in Red Deer
Steele, P1; Martin, S2; Jeffrey, M2; González, L2; Sisó, S2; Finlayson, J1;
Hamilton, S1;
Eaton, Samatha L1; Reid, Hugh W1; Todd, R1; Pang, Y1; Chianini, F1;
Dagleish, MP1
1Moredun Research Institute, UK; 2Veterinary Laboratory Agency, Lasswade, UK
Observation of clinical signs is often the first step in the diagnosis of
TSE diseases in
experimental, farmed and wild animals. Clinical presentation of chronic
wasting
disease (CWD) infected deer varies widely as disease progresses and many
clinical
signs observed can be non-specific to TSE infection, however by terminal
stage the
majority of cases involve behavioural changes and loss of body condition.
We present here the first description of clinical disease in deer
experimentally infected
with BSE. These data are part of the results of an ongoing project to
investigate the
susceptibility of UK red deer (Cervus elaphus elaphus) to BSE infection
either by
alimentary or intra-cerebral infection.
Eighteen European red deer calves (mean 64 days old) were challenged
intragastrically
with 25g of BSE-infected bovine brain. Six challenged and 2 control deer
were culled at 6 and 12 month post infection. These animals showed no
clinical signs
and no disease-specific PrP (PrPd) on immunohistochemistry (IHC) examination
of a
wide range of tissues collected at post-mortem. Six BSE-dosed and 4 negative
control
deer are still alive at time of writing (1384 dpi).
Subsequently, 6 red deer of the same cohort (mean 341 days old) were
challenged with
0.05g of BSE positive bovine brain material and 2 with sterile saline by the
intracerebral
route. Currently (1106 dpi), five of the six challenged animals have
developed
clinical signs and terminal disease confirmed by IHC and western blot
detection of
PrPd.
Clinical signs similar to CWD cases have been observed including behavioral
change,
wide stance, lowered head, and excessive salivation. All animals had
significant weight
loss attributed to inability or unwillingness to feed, with inhalation
pneumonia occurring
in the case of one animal which is commonly observed in CWD cases. The first
animal
to show clinical signs was markedly different to the four subsequent cases.
This animal had to be culled following several behavioral episodes causing
physical
injury. Our results prove for the first time that UK red deer are
susceptible to intra-cerebral
BSE infection and shows that the clinical presentation of disease shares
many
similarities to that recorded for CWD.
http://www.prion2007.com/pdf/Prion%20Bo ... tracts.pdf
M03024: Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Thursday 09 October 2003
This research project aims to determine whether BSE can be transmitted to UK
red deer by including infected material in their feed.
Study Duration: April 2003 to April 2010
Contractor: Veterinary Laboratories Agency
Background
The major cause of the spread of the BSE epidemic was attributed to the
feeding of contaminated meat and bonemeal (MBM) in the protein rations fed
to cattle. The use of MBM in animal feed was not restricted to cattle
rations and it is known that MBM was included in the concentrates fed to
farmed deer. BSE has been shown to be naturally or experimentally
transmissible to a wide range of different ungulate species and deer are
known to be susceptible to an endemic TSE (chronic wasting disease, CWD)
which is prevalent in North America. However, to date, no TSE infections of
UK deer have been reported.
Should BSE infection have been transmitted into the UK red deer population,
the CWD precedent would suggest that there is potential for both spread and
maintenance of the disease in both free living and captive UK deer
populations. The purpose of this study is to investigate the susceptibility
of UK red deer to BSE infection and to determine the clinical and
pathological phenotype.
Research Approach
The initial objective of the study is to determine whether orally infected
UK red deer are susceptible to bovine BSE agent. Groups of orally dosed deer
will be sacrificed at 6, 12 and 60 months post inoculation and necropsies
carried out. A range of tissue samples will be retained for further analysis
such as immunohistochemistry. All animals will also be monitored clinically
throughout the experiment to define any clinical phenotype.
http://www.food.gov.uk/science/research ... st/m03024/
-------- Original Message --------
Subject: Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Date: Mon, 8 Mar 2004 20:29:54 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: [email protected]
TSE Project Details
Project Ref M03024
Theme Risk assessment of SEs
Sub Theme An evaluation of SEs transmission modalities from cattle to
man and other food animals, environment vectors
MRC Priority
Title Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Funder(s)
Principle Investigator Dr Hawkins PI Department Veterinary Laboratories
Agency
PI Location Weybridge PI Organisation Veterinary Laboratories Agency
Last Year Cost £ This Year Cost £
Start Date 01/04/2003 End Date 01/04/2010
Status Current Total Cost £ 1,485,458
Abstract The major cause of the spread of BSE was attributed to the
feeding of contaminated meat and bone meal (MBM) in the protein rations
fed to cattle. The use of MBM in animal feed was not restricted to
cattle rations and it is known that MBM was included in the concentrates
fed to farmed deer. BSE has been shown to be naturally or experimentally
transmissible to a wide range of different ungulate species and deer are
known to be susceptible to an endemic TSE (chronic wasting disease, CWD)
which is prevalent in North America. However, to date, no TSE infections
of UK deer have been reported. The initial objective of the study is to
determine whether orally infected UK red deer are susceptible to bovine
BSE agent. Groups of orally dosed deer will be sacrificed at 6, 12 and
60 months post inoculation and necropsies carried out. A range of tissue
samples will be retained for further analysis such as
immunohistochemistry. All animals will also be monitored clinically
throughout the experiment to define any clinical phenotype.
©2004 Medical Research Council
http://www.mrc.ac.uk/index/current-rese ... PID=M03024
Virology
Susceptibility of Red Deer to BSE
Dagleish, M
FSA funded project in collaboration with VLA
No known cases of BSE have ever been reported in any species of deer.
However, an EU directive has decreed that provision must be made for all
ruminant species entering the human food chain to be screened for BSE and
free living and captive deer may have been exposed to the BSE agent. BSE has
affected a range of different hoof stock (domestic and exotic cattle, eland,
nyala, greater kudu, gemsbok and Arabian and scimitar-horned oryx) and
several species of cats (cheetah, puma, tiger, lion and domestic cats) by
presumed ingestion of contaminated meat and bone meal in food. As both
captive and free ranging UK deer enter the human food chain it is important
to determine their susceptibility to transmission of the BSE agent, the
nature of any possible resultant clinical disease and to develop methods of
screening deer tissues for the BSE agent to maintain the high standards of
food safety within the UK .
This study will determine in the first instance whether the BSE agent can
actually be transmitted to red deer. If this is possible the study will also
provide a description of any resultant clinical disease, pathological
changes and positive control tissue material all of which would aid
surveillance for BSE in deer within the UK .
Moredun Research Institute
Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, Scotland
Telephone - 0131 445 5111, International +44 131 445 5111,
[email protected]
Site last updated: 22 Jun 2006
http://www.mri.sari.ac.uk/vir-dagleish-proj2.asp
TSS
#################### https://lists.aegee.org/bse-l.html ####################
FULL TEXT ;
http://www.ngpc.state.ne.us/cgi-bin/ult ... 2;t=000484
http://www.ngpc.state.ne.us/cgi-bin/ult ... 2;t=000484
CREUTZFELDT JAKOB DISEASE MAD COW BASE, CWD, SCRAPIE UPDATE OCT 2007
http://cjdmadcowbaseoct2007.blogspot.com/
CJD NEWS
http://disc.server.com/Indices/236650.html
CJD VOICE (voice for _all_ victims of human TSE)
http://members.aol.com/larmstr853/cjdvoice/cjdvoice.htm
Terry S. Singeltary Sr.
P.O. Box 42
Bacliff, Texas USA 77518
Date: October 4, 2007 at 9:05 am PST
P04.80
Clinical Observations of BSE Infection in Red Deer
Steele, P1; Martin, S2; Jeffrey, M2; González, L2; Sisó, S2; Finlayson, J1;
Hamilton, S1;
Eaton, Samatha L1; Reid, Hugh W1; Todd, R1; Pang, Y1; Chianini, F1;
Dagleish, MP1
1Moredun Research Institute, UK; 2Veterinary Laboratory Agency, Lasswade, UK
Observation of clinical signs is often the first step in the diagnosis of
TSE diseases in
experimental, farmed and wild animals. Clinical presentation of chronic
wasting
disease (CWD) infected deer varies widely as disease progresses and many
clinical
signs observed can be non-specific to TSE infection, however by terminal
stage the
majority of cases involve behavioural changes and loss of body condition.
We present here the first description of clinical disease in deer
experimentally infected
with BSE. These data are part of the results of an ongoing project to
investigate the
susceptibility of UK red deer (Cervus elaphus elaphus) to BSE infection
either by
alimentary or intra-cerebral infection.
Eighteen European red deer calves (mean 64 days old) were challenged
intragastrically
with 25g of BSE-infected bovine brain. Six challenged and 2 control deer
were culled at 6 and 12 month post infection. These animals showed no
clinical signs
and no disease-specific PrP (PrPd) on immunohistochemistry (IHC) examination
of a
wide range of tissues collected at post-mortem. Six BSE-dosed and 4 negative
control
deer are still alive at time of writing (1384 dpi).
Subsequently, 6 red deer of the same cohort (mean 341 days old) were
challenged with
0.05g of BSE positive bovine brain material and 2 with sterile saline by the
intracerebral
route. Currently (1106 dpi), five of the six challenged animals have
developed
clinical signs and terminal disease confirmed by IHC and western blot
detection of
PrPd.
Clinical signs similar to CWD cases have been observed including behavioral
change,
wide stance, lowered head, and excessive salivation. All animals had
significant weight
loss attributed to inability or unwillingness to feed, with inhalation
pneumonia occurring
in the case of one animal which is commonly observed in CWD cases. The first
animal
to show clinical signs was markedly different to the four subsequent cases.
This animal had to be culled following several behavioral episodes causing
physical
injury. Our results prove for the first time that UK red deer are
susceptible to intra-cerebral
BSE infection and shows that the clinical presentation of disease shares
many
similarities to that recorded for CWD.
http://www.prion2007.com/pdf/Prion%20Bo ... tracts.pdf
M03024: Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Thursday 09 October 2003
This research project aims to determine whether BSE can be transmitted to UK
red deer by including infected material in their feed.
Study Duration: April 2003 to April 2010
Contractor: Veterinary Laboratories Agency
Background
The major cause of the spread of the BSE epidemic was attributed to the
feeding of contaminated meat and bonemeal (MBM) in the protein rations fed
to cattle. The use of MBM in animal feed was not restricted to cattle
rations and it is known that MBM was included in the concentrates fed to
farmed deer. BSE has been shown to be naturally or experimentally
transmissible to a wide range of different ungulate species and deer are
known to be susceptible to an endemic TSE (chronic wasting disease, CWD)
which is prevalent in North America. However, to date, no TSE infections of
UK deer have been reported.
Should BSE infection have been transmitted into the UK red deer population,
the CWD precedent would suggest that there is potential for both spread and
maintenance of the disease in both free living and captive UK deer
populations. The purpose of this study is to investigate the susceptibility
of UK red deer to BSE infection and to determine the clinical and
pathological phenotype.
Research Approach
The initial objective of the study is to determine whether orally infected
UK red deer are susceptible to bovine BSE agent. Groups of orally dosed deer
will be sacrificed at 6, 12 and 60 months post inoculation and necropsies
carried out. A range of tissue samples will be retained for further analysis
such as immunohistochemistry. All animals will also be monitored clinically
throughout the experiment to define any clinical phenotype.
http://www.food.gov.uk/science/research ... st/m03024/
-------- Original Message --------
Subject: Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Date: Mon, 8 Mar 2004 20:29:54 -0600
From: "Terry S. Singeltary Sr."
Reply-To: Bovine Spongiform Encephalopathy
To: [email protected]
TSE Project Details
Project Ref M03024
Theme Risk assessment of SEs
Sub Theme An evaluation of SEs transmission modalities from cattle to
man and other food animals, environment vectors
MRC Priority
Title Susceptibility of red deer (Cervus elaphus elaphus) to BSE
Funder(s)
Principle Investigator Dr Hawkins PI Department Veterinary Laboratories
Agency
PI Location Weybridge PI Organisation Veterinary Laboratories Agency
Last Year Cost £ This Year Cost £
Start Date 01/04/2003 End Date 01/04/2010
Status Current Total Cost £ 1,485,458
Abstract The major cause of the spread of BSE was attributed to the
feeding of contaminated meat and bone meal (MBM) in the protein rations
fed to cattle. The use of MBM in animal feed was not restricted to
cattle rations and it is known that MBM was included in the concentrates
fed to farmed deer. BSE has been shown to be naturally or experimentally
transmissible to a wide range of different ungulate species and deer are
known to be susceptible to an endemic TSE (chronic wasting disease, CWD)
which is prevalent in North America. However, to date, no TSE infections
of UK deer have been reported. The initial objective of the study is to
determine whether orally infected UK red deer are susceptible to bovine
BSE agent. Groups of orally dosed deer will be sacrificed at 6, 12 and
60 months post inoculation and necropsies carried out. A range of tissue
samples will be retained for further analysis such as
immunohistochemistry. All animals will also be monitored clinically
throughout the experiment to define any clinical phenotype.
©2004 Medical Research Council
http://www.mrc.ac.uk/index/current-rese ... PID=M03024
Virology
Susceptibility of Red Deer to BSE
Dagleish, M
FSA funded project in collaboration with VLA
No known cases of BSE have ever been reported in any species of deer.
However, an EU directive has decreed that provision must be made for all
ruminant species entering the human food chain to be screened for BSE and
free living and captive deer may have been exposed to the BSE agent. BSE has
affected a range of different hoof stock (domestic and exotic cattle, eland,
nyala, greater kudu, gemsbok and Arabian and scimitar-horned oryx) and
several species of cats (cheetah, puma, tiger, lion and domestic cats) by
presumed ingestion of contaminated meat and bone meal in food. As both
captive and free ranging UK deer enter the human food chain it is important
to determine their susceptibility to transmission of the BSE agent, the
nature of any possible resultant clinical disease and to develop methods of
screening deer tissues for the BSE agent to maintain the high standards of
food safety within the UK .
This study will determine in the first instance whether the BSE agent can
actually be transmitted to red deer. If this is possible the study will also
provide a description of any resultant clinical disease, pathological
changes and positive control tissue material all of which would aid
surveillance for BSE in deer within the UK .
Moredun Research Institute
Pentlands Science Park, Bush Loan, Penicuik, Midlothian, EH26 0PZ, Scotland
Telephone - 0131 445 5111, International +44 131 445 5111,
[email protected]
Site last updated: 22 Jun 2006
http://www.mri.sari.ac.uk/vir-dagleish-proj2.asp
TSS
#################### https://lists.aegee.org/bse-l.html ####################
FULL TEXT ;
http://www.ngpc.state.ne.us/cgi-bin/ult ... 2;t=000484
http://www.ngpc.state.ne.us/cgi-bin/ult ... 2;t=000484
CREUTZFELDT JAKOB DISEASE MAD COW BASE, CWD, SCRAPIE UPDATE OCT 2007
http://cjdmadcowbaseoct2007.blogspot.com/
CJD NEWS
http://disc.server.com/Indices/236650.html
CJD VOICE (voice for _all_ victims of human TSE)
http://members.aol.com/larmstr853/cjdvoice/cjdvoice.htm
Terry S. Singeltary Sr.
P.O. Box 42
Bacliff, Texas USA 77518
