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bag rot?
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<blockquote data-quote="milkmaid" data-source="post: 235745" data-attributes="member: 852"><p>You're welcome, Karen. <img src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7" class="smilie smilie--sprite smilie--sprite1" alt=":)" title="Smile :)" loading="lazy" data-shortname=":)" /></p><p></p><p>FWIW - it would be ripping you off to charge $75/culture - it only cost me $25 to have my vet run an antibiotic sensitivity test, and that had to be taken to a lab 2 hours away.</p><p></p><p>Mycoplasma plates take a little longer to grow than the others, just so you know. Takes ~7 days in the incubator compared to 18 hours for the other types of plates. So 7 days in the incubator might translate to 10 days on the counter - by the time you knew whether the infection was or was not caused by Myco the animal would either be dead or have recovered. :lol: But at least you'd know what did or did not work, even if it took awhile to get that information.</p><p></p><p>The plates can be sort of hard to read at times - I've been learning from my vet but I still can't always tell what's what. The types of plates he uses are the blood agar and MacConkie - first for gram-positives like staph/strep that live on blood, second for gram-negatives like ecoli; the plate is lactose-something or other.</p><p></p><p>The only one I can tell with ease is staph aureus on the blood agar plate. Streps and staph environmentals sort of look the same to me... (cow on the right side shows a heavy staph environmental growth - cow on the left is clean)</p><p></p><p><img src="http://img.villagephotos.com/p/2004-11/897828/cultureplateII.jpg" alt="" class="fr-fic fr-dii fr-draggable " style="" /></p><p></p><p>but s. aureus eats right through - see the right side of the plate and how you can see through it? (sorry it's not very clear!)</p><p><img src="http://img.villagephotos.com/p/2004-11/897828/cultureplateIII.jpg" alt="" class="fr-fic fr-dii fr-draggable " style="" /></p><p></p><p>I don't run samples on the MacConkie plates too often as we have more trouble with mastitis caused by staph/strep organisms.</p><p></p><p>But at least with culturing you have a better idea of what you're dealing with -- even if you can't always read exactly what's grown on the plate, you know if it's gram positive or gram negative and that does help with deciding what type of antibiotic to use.</p></blockquote><p></p>
[QUOTE="milkmaid, post: 235745, member: 852"] You're welcome, Karen. :) FWIW - it would be ripping you off to charge $75/culture - it only cost me $25 to have my vet run an antibiotic sensitivity test, and that had to be taken to a lab 2 hours away. Mycoplasma plates take a little longer to grow than the others, just so you know. Takes ~7 days in the incubator compared to 18 hours for the other types of plates. So 7 days in the incubator might translate to 10 days on the counter - by the time you knew whether the infection was or was not caused by Myco the animal would either be dead or have recovered. :lol: But at least you'd know what did or did not work, even if it took awhile to get that information. The plates can be sort of hard to read at times - I've been learning from my vet but I still can't always tell what's what. The types of plates he uses are the blood agar and MacConkie - first for gram-positives like staph/strep that live on blood, second for gram-negatives like ecoli; the plate is lactose-something or other. The only one I can tell with ease is staph aureus on the blood agar plate. Streps and staph environmentals sort of look the same to me... (cow on the right side shows a heavy staph environmental growth - cow on the left is clean) [img]http://img.villagephotos.com/p/2004-11/897828/cultureplateII.jpg[/img] but s. aureus eats right through - see the right side of the plate and how you can see through it? (sorry it's not very clear!) [img]http://img.villagephotos.com/p/2004-11/897828/cultureplateIII.jpg[/img] I don't run samples on the MacConkie plates too often as we have more trouble with mastitis caused by staph/strep organisms. But at least with culturing you have a better idea of what you're dealing with -- even if you can't always read exactly what's grown on the plate, you know if it's gram positive or gram negative and that does help with deciding what type of antibiotic to use. [/QUOTE]
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