Menu
Forums
New posts
Search forums
What's new
New posts
New media
New media comments
New profile posts
Latest activity
Media
New media
New comments
Search media
Members
Current visitors
New profile posts
Search profile posts
Log in
Register
What's new
Search
Search
Search titles and first posts only
Search titles only
By:
New posts
Search forums
Menu
Log in
Register
Forums
Cattle Boards
NCBA, R-CALF, COOL, USDA (No Politics!)
Atypical BSE (BASE) Transmitted from Cattle to Primate
JavaScript is disabled. For a better experience, please enable JavaScript in your browser before proceeding.
You are using an out of date browser. It may not display this or other websites correctly.
You should upgrade or use an
alternative browser
.
Reply to thread
Help Support CattleToday:
Message
<blockquote data-quote="flounder" data-source="post: 570660" data-attributes="member: 3519"><p>Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate </p><p></p><p></p><p> Emmanuel E. Comoy1*, Cristina Casalone2, Nathalie Lescoutra-Etchegaray1, Gianluigi Zanusso3, Sophie Freire1, Dominique Marcé1, Frédéric Auvré1, Marie-Magdeleine Ruchoux1, Sergio Ferrari3, Salvatore Monaco3, Nicole Salès4, Maria Caramelli2, Philippe Leboulch1,5, Paul Brown1, Corinne I. Lasmézas4, Jean-Philippe Deslys1</p><p></p><p>1 Institute of Emerging Diseases and Innovative Therapies, CEA, Fontenay-aux-Roses, France, 2 Istituto Zooprofilattico Sperimentale del Piemonte, Turin, Italy, 3 Policlinico G.B. Rossi, Verona, Italy, 4 Scripps Florida, Jupiter, Florida, United States of America, 5 Genetics Division, Brigham & Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America</p><p></p><p>Abstract Background Human variant Creutzfeldt-Jakob Disease (vCJD) results from foodborne transmission of prions from slaughtered cattle with classical Bovine Spongiform Encephalopathy (cBSE). Atypical forms of BSE, which remain mostly asymptomatic in aging cattle, were recently identified at slaughterhouses throughout Europe and North America, raising a question about human susceptibility to these new prion strains.</p><p></p><p>Methodology/Principal Findings Brain homogenates from cattle with classical BSE and atypical (BASE) infections were inoculated intracerebrally into cynomolgus monkeys (Macacca fascicularis), a non-human primate model previously demonstrated to be susceptible to the original strain of cBSE. The resulting diseases were compared in terms of clinical signs, histology and biochemistry of the abnormal prion protein (PrPres). The single monkey infected with BASE had a shorter survival, and a different clinical evolution, histopathology, and prion protein (PrPres) pattern than was observed for either classical BSE or vCJD-inoculated animals. Also, the biochemical signature of PrPres in the BASE-inoculated animal was found to have a higher proteinase K sensitivity of the octa-repeat region. We found the same biochemical signature in three of four human patients with sporadic CJD and an MM type 2 PrP genotype who lived in the same country as the infected bovine.</p><p></p><p>Conclusion/Significance Our results point to a possibly higher degree of pathogenicity of BASE than classical BSE in primates and also raise a question about a possible link to one uncommon subset of cases of apparently sporadic CJD. Thus, despite the waning epidemic of classical BSE, the occurrence of atypical strains should temper the urge to relax measures currently in place to protect public health from accidental contamination by BSE-contaminated products.</p><p></p><p>Citation: Comoy EE, Casalone C, Lescoutra-Etchegaray N, Zanusso G, Freire S, et al. (2008) Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate. PLoS ONE 3(8): e3017. doi:10.1371/journal.pone.0003017</p><p></p><p>Editor: Neil Mabbott, University of Edinburgh, United Kingdom</p><p></p><p>Received: April 24, 2008; Accepted: August 1, 2008; Published: August 20, 2008</p><p></p><p>Copyright: © 2008 Comoy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</p><p></p><p></p><p>snip... FULL TEXT ;</p><p></p><p><a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0003017" target="_blank">http://www.plosone.org/article/info%3Ad ... ne.0003017</a></p><p></p><p></p><p>>>> "the biochemical signature of PrPres in the BASE-inoculated animal was </p><p>found to have a higher proteinase K sensitivity of the octa-repeat </p><p>region. We found the same biochemical signature in three of four human </p><p>patients with sporadic CJD and an MM type 2 PrP genotype who lived in </p><p>the same country as the infected bovine." <<<</p><p></p><p></p><p></p><p>NOT to forget ;</p><p></p><p></p><p>Thursday, June 05, 2008</p><p></p><p>Review on the epidemiology and dynamics of BSE epidemics</p><p></p><p>Vet. Res. (2008) 39:15 <a href="http://www.vetres.org" target="_blank">www.vetres.org</a> DOI: 10.1051/vetres:2007053 c INRA, EDP Sciences, 2008 Review article</p><p></p><p>snip...</p><p></p><p>And last but not least, similarities of PrPres between Htype BSE and human prion diseases like CJD or GSS have been put forward [10], as well as between L-type BSE and CJD [17]. These findings raise questions about the origin and inter species transmission of these prion diseases that were discovered through the BSE active surveillance.</p><p></p><p>snip...</p><p></p><p>Cases of atypical BSE have only been found in countries having implemented large active surveillance programs. As of 1st September 2007, 36 cases (16 H, 20 L) have been described all over the world in cattle: Belgium (1 L) [23], Canada (1 H)15, Denmark (1 L)16, France (8 H, 6 L)17, Germany (1 H, 1 L) [13], Italy (3 L)18, Japan (1 L) [71], Netherlands (1 H, 2 L)19, Poland (1 H, 6 L)20, Sweden (1 H)21, United Kingdom (1 H)22, and USA (2 H)23. Another H-type case has been found in a 19 year old miniature zebu in a zoological park in Switzerland [56]. It is noteworthy that atypical cases have been found in countries that did not experience classical BSE so far, like Sweden, or in which only few cases of classical BSE have been found, like Canada or the USA.</p><p></p><p>And last but not least, similarities of PrPres between Htype BSE and human prion diseases like CJD or GSS have been put forward [10], as well as between L-type BSE and CJD [17]. These findings raise questions about the origin and inter species transmission of these prion diseases that were discovered through the BSE active surveillance.</p><p></p><p>full text 18 pages ;</p><p></p><p><a href="http://www.vetres.org/index.php?option=article&access=standard&Itemid=129&url=/articles/vetres/pdf/2008/04/v07232.pdf" target="_blank">http://www.vetres.org/index.php?option= ... v07232.pdf</a></p><p></p><p>please see full text ;</p><p></p><p><a href="http://bse-atypical.blogspot.com/2008/06/review-on-epidemiology-and-dynamics-of.html" target="_blank">http://bse-atypical.blogspot.com/2008/0 ... cs-of.html</a></p><p></p><p></p><p>***Atypical forms of BSE have emerged which, although rare, appear to be more virulent than the classical BSE that causes vCJD.***</p><p></p><p>Progress Report from the National Prion Disease Pathology Surveillance Center</p><p></p><p>An Update from Stephen M. Sergay, MB, BCh & Pierluigi Gambetti, MD</p><p></p><p>April 3, 2008</p><p></p><p><a href="http://www.aan.com/news/?event=read&article_id=4397&page=72.45.45" target="_blank">http://www.aan.com/news/?event=read&art ... e=72.45.45</a></p><p></p><p></p><p>Sunday, March 16, 2008</p><p></p><p>MAD COW DISEASE terminology UK c-BSE (typical), atypical BSE H or L, and or Italian L-BASE</p><p></p><p><a href="http://bse-atypical.blogspot.com/2008/03/mad-cow-disease-terminology-uk-c-bse.html" target="_blank">http://bse-atypical.blogspot.com/2008/0 ... c-bse.html</a></p><p></p><p></p><p>HUMAN and ANIMAL TSE Classifications i.e. mad cow disease and the UKBSEnvCJD only theory JUNE 2008</p><p></p><p>snip...</p><p></p><p>Tissue infectivity and strain typing of the many variants Manuscript of the human and animal TSEs are paramount in all variants of all TSE. There must be a proper classification that will differentiate between all these human TSE in order to do this. With the CDI and other more sensitive testing coming about, I only hope that my proposal will some day be taken seriously. ...</p><p></p><p>snip...</p><p></p><p><a href="http://cjdmadcowbaseoct2007.blogspot.com/2008/06/human-and-animal-tse-classifications-ie.html" target="_blank">http://cjdmadcowbaseoct2007.blogspot.co ... ns-ie.html</a></p><p></p><p></p><p></p><p>Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate </p><p></p><p><a href="http://organicconsumers.org/forum/index.php?showtopic=1951" target="_blank">http://organicconsumers.org/forum/index ... topic=1951</a></p><p></p><p><a href="http://bse-atypical.blogspot.com/2008/08/atypical-bse-base-transmitted-from.html" target="_blank">http://bse-atypical.blogspot.com/2008/0 ... -from.html</a></p><p></p><p></p><p></p><p>to be continued. ...</p><p></p><p></p><p>TSS</p></blockquote><p></p>
[QUOTE="flounder, post: 570660, member: 3519"] Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate Emmanuel E. Comoy1*, Cristina Casalone2, Nathalie Lescoutra-Etchegaray1, Gianluigi Zanusso3, Sophie Freire1, Dominique Marcé1, Frédéric Auvré1, Marie-Magdeleine Ruchoux1, Sergio Ferrari3, Salvatore Monaco3, Nicole Salès4, Maria Caramelli2, Philippe Leboulch1,5, Paul Brown1, Corinne I. Lasmézas4, Jean-Philippe Deslys1 1 Institute of Emerging Diseases and Innovative Therapies, CEA, Fontenay-aux-Roses, France, 2 Istituto Zooprofilattico Sperimentale del Piemonte, Turin, Italy, 3 Policlinico G.B. Rossi, Verona, Italy, 4 Scripps Florida, Jupiter, Florida, United States of America, 5 Genetics Division, Brigham & Women's Hospital, Harvard Medical School, Boston, Massachusetts, United States of America Abstract Background Human variant Creutzfeldt-Jakob Disease (vCJD) results from foodborne transmission of prions from slaughtered cattle with classical Bovine Spongiform Encephalopathy (cBSE). Atypical forms of BSE, which remain mostly asymptomatic in aging cattle, were recently identified at slaughterhouses throughout Europe and North America, raising a question about human susceptibility to these new prion strains. Methodology/Principal Findings Brain homogenates from cattle with classical BSE and atypical (BASE) infections were inoculated intracerebrally into cynomolgus monkeys (Macacca fascicularis), a non-human primate model previously demonstrated to be susceptible to the original strain of cBSE. The resulting diseases were compared in terms of clinical signs, histology and biochemistry of the abnormal prion protein (PrPres). The single monkey infected with BASE had a shorter survival, and a different clinical evolution, histopathology, and prion protein (PrPres) pattern than was observed for either classical BSE or vCJD-inoculated animals. Also, the biochemical signature of PrPres in the BASE-inoculated animal was found to have a higher proteinase K sensitivity of the octa-repeat region. We found the same biochemical signature in three of four human patients with sporadic CJD and an MM type 2 PrP genotype who lived in the same country as the infected bovine. Conclusion/Significance Our results point to a possibly higher degree of pathogenicity of BASE than classical BSE in primates and also raise a question about a possible link to one uncommon subset of cases of apparently sporadic CJD. Thus, despite the waning epidemic of classical BSE, the occurrence of atypical strains should temper the urge to relax measures currently in place to protect public health from accidental contamination by BSE-contaminated products. Citation: Comoy EE, Casalone C, Lescoutra-Etchegaray N, Zanusso G, Freire S, et al. (2008) Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate. PLoS ONE 3(8): e3017. doi:10.1371/journal.pone.0003017 Editor: Neil Mabbott, University of Edinburgh, United Kingdom Received: April 24, 2008; Accepted: August 1, 2008; Published: August 20, 2008 Copyright: © 2008 Comoy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. snip... FULL TEXT ; [url=http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0003017]http://www.plosone.org/article/info%3Ad ... ne.0003017[/url] >>> "the biochemical signature of PrPres in the BASE-inoculated animal was found to have a higher proteinase K sensitivity of the octa-repeat region. We found the same biochemical signature in three of four human patients with sporadic CJD and an MM type 2 PrP genotype who lived in the same country as the infected bovine." <<< NOT to forget ; Thursday, June 05, 2008 Review on the epidemiology and dynamics of BSE epidemics Vet. Res. (2008) 39:15 [url=http://www.vetres.org]www.vetres.org[/url] DOI: 10.1051/vetres:2007053 c INRA, EDP Sciences, 2008 Review article snip... And last but not least, similarities of PrPres between Htype BSE and human prion diseases like CJD or GSS have been put forward [10], as well as between L-type BSE and CJD [17]. These findings raise questions about the origin and inter species transmission of these prion diseases that were discovered through the BSE active surveillance. snip... Cases of atypical BSE have only been found in countries having implemented large active surveillance programs. As of 1st September 2007, 36 cases (16 H, 20 L) have been described all over the world in cattle: Belgium (1 L) [23], Canada (1 H)15, Denmark (1 L)16, France (8 H, 6 L)17, Germany (1 H, 1 L) [13], Italy (3 L)18, Japan (1 L) [71], Netherlands (1 H, 2 L)19, Poland (1 H, 6 L)20, Sweden (1 H)21, United Kingdom (1 H)22, and USA (2 H)23. Another H-type case has been found in a 19 year old miniature zebu in a zoological park in Switzerland [56]. It is noteworthy that atypical cases have been found in countries that did not experience classical BSE so far, like Sweden, or in which only few cases of classical BSE have been found, like Canada or the USA. And last but not least, similarities of PrPres between Htype BSE and human prion diseases like CJD or GSS have been put forward [10], as well as between L-type BSE and CJD [17]. These findings raise questions about the origin and inter species transmission of these prion diseases that were discovered through the BSE active surveillance. full text 18 pages ; [url=http://www.vetres.org/index.php?option=article&access=standard&Itemid=129&url=/articles/vetres/pdf/2008/04/v07232.pdf]http://www.vetres.org/index.php?option= ... v07232.pdf[/url] please see full text ; [url=http://bse-atypical.blogspot.com/2008/06/review-on-epidemiology-and-dynamics-of.html]http://bse-atypical.blogspot.com/2008/0 ... cs-of.html[/url] ***Atypical forms of BSE have emerged which, although rare, appear to be more virulent than the classical BSE that causes vCJD.*** Progress Report from the National Prion Disease Pathology Surveillance Center An Update from Stephen M. Sergay, MB, BCh & Pierluigi Gambetti, MD April 3, 2008 [url=http://www.aan.com/news/?event=read&article_id=4397&page=72.45.45]http://www.aan.com/news/?event=read&art ... e=72.45.45[/url] Sunday, March 16, 2008 MAD COW DISEASE terminology UK c-BSE (typical), atypical BSE H or L, and or Italian L-BASE [url=http://bse-atypical.blogspot.com/2008/03/mad-cow-disease-terminology-uk-c-bse.html]http://bse-atypical.blogspot.com/2008/0 ... c-bse.html[/url] HUMAN and ANIMAL TSE Classifications i.e. mad cow disease and the UKBSEnvCJD only theory JUNE 2008 snip... Tissue infectivity and strain typing of the many variants Manuscript of the human and animal TSEs are paramount in all variants of all TSE. There must be a proper classification that will differentiate between all these human TSE in order to do this. With the CDI and other more sensitive testing coming about, I only hope that my proposal will some day be taken seriously. ... snip... [url=http://cjdmadcowbaseoct2007.blogspot.com/2008/06/human-and-animal-tse-classifications-ie.html]http://cjdmadcowbaseoct2007.blogspot.co ... ns-ie.html[/url] Atypical BSE (BASE) Transmitted from Asymptomatic Aging Cattle to a Primate [url=http://organicconsumers.org/forum/index.php?showtopic=1951]http://organicconsumers.org/forum/index ... topic=1951[/url] [url=http://bse-atypical.blogspot.com/2008/08/atypical-bse-base-transmitted-from.html]http://bse-atypical.blogspot.com/2008/0 ... -from.html[/url] to be continued. ... TSS [/QUOTE]
Insert quotes…
Verification
Post reply
Forums
Cattle Boards
NCBA, R-CALF, COOL, USDA (No Politics!)
Atypical BSE (BASE) Transmitted from Cattle to Primate
Top