Ranchers in Texas Need to Pay Close Attention to CWD TSE Prion

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If it transmits to pigs by oral route, maybe it is already in the dadgummed feral hogs.

Price of TSE Prion Poker goes up substantially, all you cattle ranchers and such, better pay close attention here...terry

Transmission of the chronic wasting disease agent from elk to cattle after oronasal exposure

Justin Greenlee, Jifeng Bian, Zoe Lambert, Alexis Frese, and Eric Cassmann Virus and Prion Research Unit, National Animal Disease Center, USDA-ARS, Ames, IA, USA

Aims: The purpose of this study was to determine the susceptibility of cattle to chronic wasting disease agent from elk.

Conclusions: Cattle with the E211K polymorphism are susceptible to the CWD agent after oronasal exposure of 0.2 g of infectious material.

"Cattle with the E211K polymorphism are susceptible to the CWD agent after oronasal exposure of 0.2 g of infectious material."

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Strain characterization of chronic wasting disease in bovine-PrP transgenic mice

Conclusions: Altogether, these results exhibit the diversity of CWD strains present in the panel of CWD isolates and the ability of at least some CWD isolates to infect bovine species. Cattle being one of the most important farming species, this ability represents a potential threat to both animal and human health, and consequently deserves further study.

"Altogether, these results exhibit the diversity of CWD strains present in the panel of CWD isolates and the ability of at least some CWD isolates to infect bovine species. Cattle being one of the most important farming species, this ability represents a potential threat to both animal and human health, and consequently deserves further study."

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https://prion2023.org/wp-content/uploads/2023/10/Meeting-book-final-version2.pdf



cwd scrapie pigs oral routes

***> However, at 51 months of incubation or greater, 5 animals were positive by one or more diagnostic methods. Furthermore, positive bioassay results were obtained from all inoculated groups (oral and intracranial; market weight and end of study) suggesting that swine are potential hosts for the agent of scrapie. <***

>*** Although the current U.S. feed ban is based on keeping tissues from TSE infected cattle from contaminating animal feed, swine rations in the U.S. could contain animal derived components including materials from scrapie infected sheep and goats. These results indicating the susceptibility of pigs to sheep scrapie, coupled with the limitations of the current feed ban, indicates that a revision of the feed ban may be necessary to protect swine production and potentially human health. <***

***> Results: PrPSc was not detected by EIA and IHC in any RPLNs. All tonsils and MLNs were negative by IHC, though the MLN from one pig in the oral <6 month group was positive by EIA. PrPSc was detected by QuIC in at least one of the lymphoid tissues examined in 5/6 pigs in the intracranial <6 months group, 6/7 intracranial >6 months group, 5/6 pigs in the oral <6 months group, and 4/6 oral >6 months group. Overall, the MLN was positive in 14/19 (74%) of samples examined, the RPLN in 8/18 (44%), and the tonsil in 10/25 (40%).

***> Conclusions: This study demonstrates that PrPSc accumulates in lymphoid tissues from pigs challenged intracranially or orally with the CWD agent, and can be detected as early as 4 months after challenge. CWD-infected pigs rarely develop clinical disease and if they do, they do so after a long incubation period. This raises the possibility that CWD-infected pigs could shed prions into their environment long before they develop clinical disease. Furthermore, lymphoid tissues from CWD-infected pigs could present a potential source of CWD infectivity in the animal and human food chains.

https://www.ars.usda.gov/research/publications/publication/?seqNo115=353091

https://www.ars.usda.gov/research/project/?accnNo=432011&fy=2017

https://www.ars.usda.gov/research/publications/publication/?seqNo115=337105


Conclusions: This study demonstrates that PrPSc accumulates in lymphoid tissues from pigs challenged intracranially or orally with the CWD agent, and can be detected as early as 4 months after challenge. CWD-infected pigs rarely develop clinical disease and if they do, they do so after a long incubation period. This raises the possibility that CWD-infected pigs could shed prions into their environment long before they develop clinical disease. Furthermore, lymphoid tissues from CWD-infected pigs could present a potential source of CWD infectivity in the animal and human food chains.

https://www.ars.usda.gov/research/publications/publication/?seqNo115=337105

THIS MUST CHANGE ASAP!

"For elk and deer considered at high risk for CWD, the FDA recommends that these animals do not enter the animal feed system. However, this recommendation is guidance and not a requirement by law."…

Snip…

please see my full submission with reference materials… 300NG is enough to transmit cwd to deer by oral route!

Monday, November 13, 2023

Food and Drug Administration's BSE Feed Regulation (21 CFR 589.2000) Singeltary Another Request for Update 2023

https://fdabse589.blogspot.com/2023/11/food-and-drug-administrations-bse-feed.htm

THIS MUST CHANGE ASAP!

"For elk and deer considered at high risk for CWD, the FDA recommends that these animals do not enter the animal feed system. However, this recommendation is guidance and not a requirement by law."…

Snip…

please see my full submission with reference materials… 300NG is enough to transmit cwd to deer by oral route!

Monday, November 13, 2023

Food and Drug Administration's BSE Feed Regulation (21 CFR 589.2000) Singeltary Another Request for Update 2023

https://fdabse589.blogspot.com/2023/11/food-and-drug-administrations-bse-feed.htm

terry
 

spot on! i have been very concerned about semen and cwd for over a decade.

PLEASE NOTE, those straw bred $25k bucks, and everything that goes with it, what I have called for years "jerking for dollars, and possibly CWD", where the industry has gotten so risky, it trades sperm fro Cervid like kids trade baseball cards, or lovers swap spit, even politicians use high dollar straw bred Buck sperm for political donations, might just come back to haunt everyone wit a CWD…what if? I DON'T MAKE THIS STUFF UP! WAKE UP PEOPLE!

PMCA successfully detected CWD-prions in a diverse array of samples including blood, semen, feces, obex, retropharyngeal lymph node, fetuses (neural and peripheral tissues) and gestational tissues, parasites-insects, plants, compost-soil mixtures, and swabs from trash containers.

https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true

Published: 15 September 2021

Detection of CWD prions in naturally infected white-tailed deer fetuses and gestational tissues by PMCA

Francisca Bravo-Risi, Paulina Soto, Thomas Eckland, Robert Dittmar, Santiago Ramírez, Celso S. G. Catumbela, Claudio Soto, Mitch Lockwood, Tracy Nichols & Rodrigo Morales Scientific Reports volume 11, Article number: 18385 (2021) Cite this article

This data confirms previous findings in other animal species and furthers vertical transmission as a relevant mechanism of CWD dissemination.

Snip…

Here, we report the presence of seeding competent CWD prions in fetal tissues collected from naturally prion-infected farmed WTD does using PMCA. The results presented in this article confirm the presence of CWD prions in fetal tissues from naturally infected farmed WTD dams suggesting that CWD could be transferred from mother to offspring.

snip...

https://www.nature.com/articles/s41598-021-97737-y

14 Protein misfolding cyclic amplification (PMCA) as an ultra-sensitive technique for the screening of CWD prions in different sample types.

Abstract

PMCA successfully detected CWD-prions in a diverse array of samples including blood, semen, feces, obex, retropharyngeal lymph node, fetuses (neural and peripheral tissues) and gestational tissues, parasites-insects, plants, compost-soil mixtures, and swabs from trash containers.

Importantly, our findings identified CWD in areas previously considered to be free of CWD. Overall, our findings demonstrate that PMCA is a powerful technique for the screening of biological and environmental samples, and it may prove useful as a CWD management and surveillance tool.

https://intcwdsympo.files.wordpress.com/2023/06/final-agenda-with-abstracts.pdf?force_download=true

P74 High Prevalence of CWD prions in male reproductive samples

The results showed positive CWD prion detection in testes, epididymis and seminal fluid samples. A high prevalence of CWD-PrPSc was found in samples collected at the late-presymptomatic stage of the disease. Our results showed a correlation between the presence of CWD-PrPSc in male reproductive organs and blood. These findings demonstrate a high efficiency of CWD prion detection by PMCA in testes, epididymis and seminal fluid, and offer a possibility for a routine screening of semen samples to be commercially distributed for artificial insemination. Our results may uncover new opportunities to understand the mechanisms of CWD spreading and decrease putative inter-individual transmission associated to insemination using CWD contaminated specimens.

https://web.archive.org/web/2019103...18.org/wp-content/uploads/2018/05/program.pdf

In Vitro detection of Chronic Wasting Disease (CWD) prions in semen and reproductive tissues of white tailed deer bucks (Odocoileus virginianus)

Our findings reveal the presence of CWD prions in semen and sexual tissues of prion infected WTD bucks. Future studies will be necessary to determine whether sexual contact and/or artificial inseminations are plausible means of CWD transmission in susceptible animal species.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936793/

Very low oral exposure to prions of brain or saliva origin can transmit chronic wasting disease

We report that oral exposure to as little as 300 nanograms (ng) of CWD-positive brain or to saliva containing seeding activity equivalent to 300 ng of CWD-positive brain, were sufficient to transmit CWD disease. This was true whether the inoculum was administered as a single bolus or divided as three weekly 100 ng exposures. However, when the 300 ng total dose was apportioned as 10, 30 ng doses delivered over 12 weeks, no infection occurred. While low-dose exposures to prions of brain or saliva origin prolonged the time from inoculation to first detection of infection, once infection was established, we observed no differences in disease pathogenesis. These studies suggest that the CWD minimum infectious dose approximates 100 to 300 ng CWD-positive brain (or saliva equivalent), and that CWD infection appears to conform more with a threshold than a cumulative dose dynamic.

https://pubmed.ncbi.nlm.nih.gov/32817706/

Study raises possibility of sexual spread of CWD in deer

https://www.cidrap.umn.edu/chronic-wasting-disease/study-raises-possibility-sexual-spread-cwd-deer

SUNDAY, FEBRUARY 16, 2020

***> Jerking for Dollars, Are Texas Politicians and Legislators Masturbating Deer For Money, and likely spreading CWD TSE Prion?

https://chronic-wasting-disease.blogspot.com/2020/02/jerking-for-dollars-are-texas.html

TUESDAY, DECEMBER 31, 2019

In Vitro detection of Chronic Wasting Disease (CWD) prions in semen and reproductive tissues of white tailed deer bucks (Odocoileus virginianus

https://chronic-wasting-disease.blogspot.com/2019/12/in-vitro-detection-of-chronic-wasting.html

WEDNESDAY, MARCH 13, 2019

CWD, TSE, PRION, MATERNAL mother to offspring, testes, epididymis, seminal fluid, and blood

https://chronic-wasting-disease.blogspot.com/2019/03/cwd-tse-prion-maternal-mother-to.html

SUNDAY, AUGUST 02, 2015

TEXAS CWD, Have you been ThunderStruck, deer semen, straw bred bucks, super ovulation, and the potential TSE Prion connection, what if?

https://chronic-wasting-disease.blogspot.com/2015/08/texas-cwd-have-you-been-thunderstruck.html

SUNDAY, AUGUST 02, 2015

http://chronic-wasting-disease.blogspot.com/2015/08/texas-cwd-have-you-been-thunderstruck.html

terry
 
BSE ≠ CWD
Topic of the thread is chronic wasting disease and its transmissibility to animals other than deer.
 
Last edited:
That would be very important to find out. Here is a study researched that topic, first and last paragraph.
They let cattle graze in pens with CWB infected deer and elk for 10 years. Also gave some cattle large oral doses of CWD infected mule deer brains and also watched over those animals for 10 years. Shockingly, some cattle (not all), did developed BSE-like disease 2 to 5 years after CWD brain material was injected directly into their brains

Non Technical Summary
CWD is a transmissible spongiform encephalopathy that infects wild and farmed deer and elk. It is related to the prion diseases scrapie of sheep and BSE of cattle. It has been shown that some cattle can develop a BSE-like disease 2 to 5 years afterwards when this CWD brain material is injected intracerebrally, but it is not known if a more natural route of exposure to the infectious agent can transmit the disease to cattle. Since cattle can encounter CWD-infected deer and/or elk, or the long-lasting infectious prion shed by them, on shared grazing range, a realistic assessment of the possibility they could become infected is needed. The BSE epidemic in the UK in the 1980's and subsequent transmission of the BSE prion to humans through infected beef products created an awareness of the possible economic and public health impacts of a similar occurrence in the U.S. This research seeks to determine if domestic cattle that have been kept in physical contact with chronic wasting disease-infected deer and elk or with contaminated premises for ten years also became infected. This research project will analyze brain and other tissues from the CWD-exposed cattle, none of which have died or developed clinical signs of disease, to determine if transmission has occurred. A separate project will examine cattle were given a large oral dose of macerated brain tissues from CWD-infected mule deer ten years ago. Whether CWD in cattle would become infectious for humans is not known, but it is a risk factor that would need to be evaluated.


Impacts
This study investigated the risk of domestic cattle developing a transmissible spongiform encephalopathy (TSE) after oral inoculation followed by a long incubation period, or by long-term natural exposure to cervids infected with chronic wasting disease (CWD). Previously,ten cattle were given large oral doses of pooled brain material from CWD-infected mule deer in late August 1997 and housed in isolation at the Wyoming State Veterinary Laboratory until September 2007. Two additional groups of cattle were penned outdoors with CWD-infected deer and elk or in CWD-contaminated premises at Colorado Division of Wildlife (n=11) and Wyoming Game and Fish Department (n=10) research facilities during the same ten-year period. These conditions simulated exposure routes that cattle in North America might encounter if they are raised or grazed in areas where free-ranging or captive deer and elk are infected with CWD. During the current project, all exposed and three untreated control cattle were killed, and select tissues were collected at necropsy. Samples from each animal were analyzed for the diagnostic hallmarks of TSEs by histological examination, immunohistochemistry and Western blot. DNA sequences were determined for the cellular prion protein gene in each animal. No proteinase-K resistant prion protein or anti-PrP immunoreactive IHC signals were detected in any tissues of exposed or control animals. None of these results, taken individually or together, support a diagnosis of TSE in cattle inoculated orally with a high dose of infectious CWD material or continually exposed by cohabitation with infected deer or elk, or transmission from contaminated premises despite an incubation period of up to 10 or 11 years.

 

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