Embryo’s…?

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Redgully

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Simme, I agree. However, after flushing embryos they should be examined under a microscope to determine the stage. The stages are 1 - 9, but viable embryos are stage 4, 5, 6 or 7. The stage is determined by how much the embryo has grown and not necessarily how many days it was before the donor cow was flushed. It is important to know the stage of your embryos so they are implanted the correct number of days after standing heat.
Here we just have grade one and grade two embryos. Grade one can be frozen but grade two go straight into donors at time of flushing. I find the stage grading quite interesting, might need to look into that more. When i was involved with flushing a cow we had over 20 structures but ended up with 6 frozen and 3 grade 2 that went straight into donors.
 

gizmom

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We did our ET work on Sunday set up 19 put eggs in 15 now wait to see who recycles. We put one egg in that we flushed out of a 13 year old cow back in 2013 the mating was to my all time favorite bull Coleman EXT 6149 I just loved that bull. I also AI’d a few back to him this year the good ones never get stale.

I saw the comment about not freezing grade 2, we have had excellent success freezing and implanting grade 2 eggs I don’t freeze grade 3 but have used them direct transfer with some success not great but have had them stick.
 

J+ Cattle

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When i was involved with flushing a cow we had over 20 structures but ended up with 6 frozen and 3 grade 2 that went straight into donors.
I'm not sure I understand when you said you had "over 20 structures", what do you mean? Is it over 20 eggs recovered but only 9 that were viable for transplanting?
 

Redgully

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I'm not sure I understand when you said you had "over 20 structures", what do you mean? Is it over 20 eggs recovered but only 9 that were viable for transplanting?
Yes that is correct, it was the cows first flush and we went with a standard hormone set up. The vet said she was a little over stimulated so some eggs released were either not fertilised or not developed. On her next flush he can tweak the program now he knows how she responds.
 

J+ Cattle

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On her next flush he can tweak the program now he knows how she responds.
I find this an interesting topic so please pardon my questions and ignorance.
When you flush her and remove the eggs there are no eggs left to attach to the uterus wall and so she will recycle again in 18-24 days. Can you flush her again that soon or is it advisable to wait longer?
 

moses388

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Earlier in the thread I posted a link to a pdf file. I am no expert on embryos, but here is what it says on grade/quality numbers. page 31/32 - Grade/quality numbers are 1-4. They refer to the composition and quantity of debris that is not part of the main cell mass.

To rephrase -
When a fertilized egg grows, the cells divide or split in half. This creates 2 cells where there was only 1 cell; these are called blastomere. Some blastomeres remain part of the main cell mass, but others may travel away from the main cell and are called extruded blastomeres (debris). Extruded blastomeres are visible under a microscope. The grade reflects the quantity of extruded blastomeres. A grade of 1 means there are no extruded blastomeres. A grade of 4 means there are numerous extruded blastomeres. The quantity of debris does not affect the viability of the embryo.
 

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