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NCBA, R-CALF, COOL, USDA (No Politics!)
BSE, SCRAPIE, CWD, TSE PRION END OF YEAR REPORTS USA AND CANADA
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<blockquote data-quote="flounder" data-source="post: 1470376" data-attributes="member: 3519"><p>Detection of PrPBSE and prion infectivity in the ileal Peyer's patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy</p><p></p><p>Ivett Ackermann, Anne Balkema-Buschmann, Reiner Ulrich, Kerstin Tauscher, James C. Shawulu, Markus Keller, Olanrewaju I. Fatola, Paul Brown and Martin H. GroschupEmail authorView ORCID ID profile</p><p></p><p>Veterinary Research201748:88</p><p></p><p><a href="https://doi.org/10.1186/s13567-017-0495-5" target="_blank">https://doi.org/10.1186/s13567-017-0495-5</a>© The Author(s) 2017</p><p></p><p>Received: 22 August 2017Accepted: 1 December 2017Published: 19 December 2017</p><p></p><p>Abstract</p><p></p><p>In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer's patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4–6 months of age were orally challenged, while there are strong indications that the risk of infection is highest in young animals. In the present study, unweaned calves aged 4–6 weeks were orally challenged to determine the earliest time point at which newly formed PrPBSE and BSE infectivity are detectable in the IPP. For this purpose, calves were culled 1 week as well as 2, 4, 6 and 8 months post-infection (mpi) and IPPs were examined for BSE infectivity using a bovine PrP transgenic mouse bioassay, and for PrPBSE by immunohistochemistry (IHC) and protein misfolding cyclic amplification (PMCA) assays. For the first time, BSE prions were detected in the IPP as early as 2 mpi by transgenic mouse bioassay and PMCA and 4 mpi by IHC in the follicular dendritic cells (FDCs) of the IPP follicles. These data indicate that BSE prions propagate in the IPP of unweaned calves within 2 months of oral uptake of the agent.</p><p></p><p>snip...</p><p></p><p>In summary, our study demonstrates for the first time PrPBSE (by PMCA) and prion infectivity (by mouse bioassay) in the ileal Peyer's patch (IPP) of young calves as early as 2 months after infection. From 4 mpi nearly all calves showed PrPBSE positive IPP follicles (by IHC), even with PrPBSE accumulation detectable in FDCs in some animals. Finally, our results confirm the IPP as the early port of entry for the BSE agent and a site of initial propagation of PrPBSE and infectivity during the early pathogenesis of the disease. Therefore, our study supports the recommendation to remove the last four metres of the small intestine (distal ileum) at slaughter, as designated by current legal requirements for countries with a controlled BSE risk status, as an essential measure for consumer and public health protection.</p><p></p><p><a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0495-5" target="_blank">https://veterinaryresearch.biomedcentra ... 017-0495-5</a></p><p></p><p>Thursday, November 16, 2017 </p><p></p><p>Texas Natural Meats Recalls Beef Products Due To Possible Specified Risk Materials Contamination</p><p></p><p><a href="http://specifiedriskmaterial.blogspot.com/2017/11/texas-natural-meats-recalls-beef.html" target="_blank">http://specifiedriskmaterial.blogspot.c ... -beef.html</a></p><p></p><p>FRIDAY, DECEMBER 22, 2017 </p><p></p><p>Detection of PrPBSE and prion infectivity in the ileal Peyer's patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy</p><p></p><p><a href="http://bovineprp.blogspot.com/2017/12/detection-of-prpbse-and-prion.html" target="_blank">http://bovineprp.blogspot.com/2017/12/d ... prion.html</a></p></blockquote><p></p>
[QUOTE="flounder, post: 1470376, member: 3519"] Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy Ivett Ackermann, Anne Balkema-Buschmann, Reiner Ulrich, Kerstin Tauscher, James C. Shawulu, Markus Keller, Olanrewaju I. Fatola, Paul Brown and Martin H. GroschupEmail authorView ORCID ID profile Veterinary Research201748:88 [url=https://doi.org/10.1186/s13567-017-0495-5]https://doi.org/10.1186/s13567-017-0495-5[/url]© The Author(s) 2017 Received: 22 August 2017Accepted: 1 December 2017Published: 19 December 2017 Abstract In classical bovine spongiform encephalopathy (C-BSE), an orally acquired prion disease of cattle, the ileal Peyer’s patch (IPP) represents the main entry port for the BSE agent. In earlier C-BSE pathogenesis studies, cattle at 4–6 months of age were orally challenged, while there are strong indications that the risk of infection is highest in young animals. In the present study, unweaned calves aged 4–6 weeks were orally challenged to determine the earliest time point at which newly formed PrPBSE and BSE infectivity are detectable in the IPP. For this purpose, calves were culled 1 week as well as 2, 4, 6 and 8 months post-infection (mpi) and IPPs were examined for BSE infectivity using a bovine PrP transgenic mouse bioassay, and for PrPBSE by immunohistochemistry (IHC) and protein misfolding cyclic amplification (PMCA) assays. For the first time, BSE prions were detected in the IPP as early as 2 mpi by transgenic mouse bioassay and PMCA and 4 mpi by IHC in the follicular dendritic cells (FDCs) of the IPP follicles. These data indicate that BSE prions propagate in the IPP of unweaned calves within 2 months of oral uptake of the agent. snip... In summary, our study demonstrates for the first time PrPBSE (by PMCA) and prion infectivity (by mouse bioassay) in the ileal Peyer’s patch (IPP) of young calves as early as 2 months after infection. From 4 mpi nearly all calves showed PrPBSE positive IPP follicles (by IHC), even with PrPBSE accumulation detectable in FDCs in some animals. Finally, our results confirm the IPP as the early port of entry for the BSE agent and a site of initial propagation of PrPBSE and infectivity during the early pathogenesis of the disease. Therefore, our study supports the recommendation to remove the last four metres of the small intestine (distal ileum) at slaughter, as designated by current legal requirements for countries with a controlled BSE risk status, as an essential measure for consumer and public health protection. [url=https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-017-0495-5]https://veterinaryresearch.biomedcentra ... 017-0495-5[/url] Thursday, November 16, 2017 Texas Natural Meats Recalls Beef Products Due To Possible Specified Risk Materials Contamination [url=http://specifiedriskmaterial.blogspot.com/2017/11/texas-natural-meats-recalls-beef.html]http://specifiedriskmaterial.blogspot.c ... -beef.html[/url] FRIDAY, DECEMBER 22, 2017 Detection of PrPBSE and prion infectivity in the ileal Peyer’s patch of young calves as early as 2 months after oral challenge with classical bovine spongiform encephalopathy [url=http://bovineprp.blogspot.com/2017/12/detection-of-prpbse-and-prion.html]http://bovineprp.blogspot.com/2017/12/d ... prion.html[/url] [/QUOTE]
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