Experimental H-type bovine spongiform encephalopathy characterized by plaques and glial- and stellate-type prion protein deposits
Experimental H-type bovine spongiform encephalopathy characterized by plaques and glial- and stellate-type prion protein deposits
Hiroyuki Okada1*, Yoshifumi Iwamaru1, Morikazu Imamura1, Kentaro Masujin1, Yuichi Matsuura1, Yoshihisa Shimizu1 , Kazuo Kasai1, Shirou Mohri1, Takashi Yokoyama1 and Stefanie Czub2
Abstract
Atypical bovine spongiform encephalopathy (BSE) has recently been identified in Europe, North America, and Japan. It is classified as H-type and L-type BSE according to the molecular mass of the disease-associated prion protein (PrPSc). To investigate the topographical distribution and deposition patterns of immunolabeled PrPSc, Htype BSE isolate was inoculated intracerebrally into cattle. H-type BSE was successfully transmitted to 3 calves, with incubation periods between 500 and 600 days. Moderate to severe spongiform changes were detected in the cerebral and cerebellar cortices, basal ganglia, thalamus, and brainstem. H-type BSE was characterized by the presence of PrP-immunopositive amyloid plaques in the white matter of the cerebrum, basal ganglia, and thalamus. Moreover, intraglial-type immunolabeled PrPSc was prominent throughout the brain. Stellate-type immunolabeled PrPSc was conspicuous in the gray matter of the cerebral cortex, basal ganglia, and thalamus, but not in the brainstem. In addition, PrPSc accumulation was detected in the peripheral nervous tissues, such as trigeminal ganglia, dorsal root ganglia, optic nerve, retina, and neurohypophysis. Cattle are susceptible to H-type BSE with a shorter incubation period, showing distinct and distinguishable phenotypes of PrPSc accumulation.
snip...
The present study demonstrated successful intraspecies transmission of H-type BSE to cattle and the distribution and immunolabeling patterns of PrPSc in the brain of the H-type BSE-challenged cattle. TSE agent virulence can be minimally defined by oral transmission of different TSE agents (C-type, L-type, and H-type BSE agents) [59]. Oral transmission studies with H-type BSEinfected cattle have been initiated and are underway to provide information regarding the extent of similarity in the immunohistochemical and molecular features before and after transmission.
In addition, the present data will support risk assessments in some peripheral tissues derived from cattle affected with H-type BSE.
http://www.veterinaryresearch.org/conte ... -42-79.pdf
Friday, May 11, 2012
Experimental H-type bovine spongiform encephalopathy characterized by plaques and glial- and stellate-type prion protein deposits
http://transmissiblespongiformencephalo ... iform.html
IN the link below, you can find California mad cow feed ban warning letter and many, many more. tonnage of mad cow feed in commerce 2000-2011, SRM, FEED, and surveillance breaches. to see the feed ban warning letters, skroll down a bit to get to them.......terry
Friday, May 18, 2012
Update from APHIS Regarding a Detection of Bovine Spongiform Encephalopathy (BSE) in the United States Friday May 18, 2012
http://transmissiblespongiformencephalo ... ction.html
================
can someone here please show everyone the test the USDA et al used to show this l-type atypical base bse was NOT from feed ???
kind regards,
terry
Experimental H-type bovine spongiform encephalopathy characterized by plaques and glial- and stellate-type prion protein deposits
Hiroyuki Okada1*, Yoshifumi Iwamaru1, Morikazu Imamura1, Kentaro Masujin1, Yuichi Matsuura1, Yoshihisa Shimizu1 , Kazuo Kasai1, Shirou Mohri1, Takashi Yokoyama1 and Stefanie Czub2
Abstract
Atypical bovine spongiform encephalopathy (BSE) has recently been identified in Europe, North America, and Japan. It is classified as H-type and L-type BSE according to the molecular mass of the disease-associated prion protein (PrPSc). To investigate the topographical distribution and deposition patterns of immunolabeled PrPSc, Htype BSE isolate was inoculated intracerebrally into cattle. H-type BSE was successfully transmitted to 3 calves, with incubation periods between 500 and 600 days. Moderate to severe spongiform changes were detected in the cerebral and cerebellar cortices, basal ganglia, thalamus, and brainstem. H-type BSE was characterized by the presence of PrP-immunopositive amyloid plaques in the white matter of the cerebrum, basal ganglia, and thalamus. Moreover, intraglial-type immunolabeled PrPSc was prominent throughout the brain. Stellate-type immunolabeled PrPSc was conspicuous in the gray matter of the cerebral cortex, basal ganglia, and thalamus, but not in the brainstem. In addition, PrPSc accumulation was detected in the peripheral nervous tissues, such as trigeminal ganglia, dorsal root ganglia, optic nerve, retina, and neurohypophysis. Cattle are susceptible to H-type BSE with a shorter incubation period, showing distinct and distinguishable phenotypes of PrPSc accumulation.
snip...
The present study demonstrated successful intraspecies transmission of H-type BSE to cattle and the distribution and immunolabeling patterns of PrPSc in the brain of the H-type BSE-challenged cattle. TSE agent virulence can be minimally defined by oral transmission of different TSE agents (C-type, L-type, and H-type BSE agents) [59]. Oral transmission studies with H-type BSEinfected cattle have been initiated and are underway to provide information regarding the extent of similarity in the immunohistochemical and molecular features before and after transmission.
In addition, the present data will support risk assessments in some peripheral tissues derived from cattle affected with H-type BSE.
http://www.veterinaryresearch.org/conte ... -42-79.pdf
Friday, May 11, 2012
Experimental H-type bovine spongiform encephalopathy characterized by plaques and glial- and stellate-type prion protein deposits
http://transmissiblespongiformencephalo ... iform.html
IN the link below, you can find California mad cow feed ban warning letter and many, many more. tonnage of mad cow feed in commerce 2000-2011, SRM, FEED, and surveillance breaches. to see the feed ban warning letters, skroll down a bit to get to them.......terry
Friday, May 18, 2012
Update from APHIS Regarding a Detection of Bovine Spongiform Encephalopathy (BSE) in the United States Friday May 18, 2012
http://transmissiblespongiformencephalo ... ction.html
================
can someone here please show everyone the test the USDA et al used to show this l-type atypical base bse was NOT from feed ???
kind regards,
terry
