Oh yes, they do. Any brucellosis free state will require it if cattle are coming from a non-free state. As far as brucellosis (Bang's) vaccination is concerned, it isn't a bad idea to continue if you forsee exporting cattle. California comes to mind (not midwestern, I know) as they absolutely require it for breeding age females to be imported, unless they are too young or are of the age they can still be vaccinated after import.
If you are interested in state import requirements go to:
http://www.aphis.usda.gov/import_export ... ates.shtml
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